• Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using NRF1 antibody (STJ27493) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Western blot analysis of various lysates using NRF1 Rabbit polyclonal antibody (STJ27493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NRF1 antibody (240-449) (STJ27493)

SKU:
STJ27493

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA/ChIP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
ChIP:5 Mu g antibody for 10 Mu g-15 Mu g of Chromatin
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRF1
Gene ID: 4899
Uniprot ID: NRF1_HUMAN
Immunogen Region: 240-449
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1).
Immunogen Sequence: WANVRSDVRTEEQKQRVSWT QALRTIVKNCYKQHGREDLL YAFEDQQTQTQATATHSIAH LVPSQTVVQTFSNPDGTVSL IQVGTGATVATLADASELPT TVTVAQVNYSAVADGEVEQN WATLQGGEMTIQTTQASEAT QAVASLAEAAVAASQEMQQG ATVTMALNSEAAAHAVATLA EATLQGGGQIVLSGETAAAV GALTGVQDAN
Tissue Specificity Ubiquitously expressed with strongest expression in skeletal muscle.
Post Translational Modifications Phosphorylation enhances DNA binding.
Function Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Protein Name Nuclear Respiratory Factor 1
Nrf-1
Alpha Palindromic-Binding Protein
Alpha-Pal
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Respiratory Factor 1 antibody
Anti-Nrf-1 antibody
Anti-Alpha Palindromic-Binding Protein antibody
Anti-Alpha-Pal antibody
Anti-NRF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance