Western blot analysis of extracts of various cell lines, using NPL antibody (STJ113623) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NPL antibody (1-250) (STJ113623)

SKU:
STJ113623
£112.50 - £524.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPL
Gene ID: 80896
Uniprot ID: NPL_HUMAN
Immunogen Region: 1-250
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NPL (NP_110396.1).
Immunogen Sequence: MAFPKKKLQGLVAATITPMT ENGEINFSVIGQYVDYLVKE QGVKNIFVNGTTGEGLSLSV SERRQVAEEWVTKGKDKLDQ VIIHVGALSLKESQELAQHA AEIGADGIAVIAPFFLKPWT KDILINFLKEVAAAAPALPF YYYHIPALTGVKIRAEELLD GILDKIPTFQGLKFSDTDLL DFGQCVDQNRQQQFAFLFGV DEQLLSALVMGATGAVGSTY NYLGKKTNQMLEAFEQKDF
Tissue Specificity Isoform 2 is expressed in placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine and peripheral blood leukocyte.
Function Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (Probable).
Protein Name N-Acetylneuraminate Lyase
Nalase
N-Acetylneuraminate Pyruvate-Lyase
N-Acetylneuraminic Acid Aldolase
Sialate Lyase
Sialate-Pyruvate Lyase
Sialic Acid Aldolase
Sialic Acid Lyase
Database Links Reactome: R-HSA-4085001
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-N-Acetylneuraminate Lyase antibody
Anti-Nalase antibody
Anti-N-Acetylneuraminate Pyruvate-Lyase antibody
Anti-N-Acetylneuraminic Acid Aldolase antibody
Anti-Sialate Lyase antibody
Anti-Sialate-Pyruvate Lyase antibody
Anti-Sialic Acid Aldolase antibody
Anti-Sialic Acid Lyase antibody
Anti-NPL antibody
Anti-C1orf13 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJA0009408 Anti-NPL antibody (STJA0009408) Rabbit Polyclonal Human/Mouse/Rat WB
STJ117118 Anti-XPNPEP3 antibody (1-250) (STJ117118) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
STJ117537 Anti-SNX4 antibody (1-250) (STJ117537) Rabbit Polyclonal Human/Mouse WB/ELISA
STJ117804 Anti-SUGCT antibody (1-250) (STJ117804) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ117471 Anti-GUCY1B3 antibody (1-250) (STJ117471) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ116006 Anti-ZNF195 antibody (1-250) (STJ116006) Rabbit Polyclonal Human/Mouse WB/ELISA
STJ118650 Anti-ALOX12B antibody (1-250) (STJ118650) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ29810 Anti-YES1 antibody (1-250) (STJ29810) Rabbit Polyclonal Human/Mouse WB/IF/ICC/ELISA
!