• Western blot analysis of extracts from wild type (WT) and NPHS1 knockdown (KD) A-549 cells, using NPHS1 antibody (STJ11104633) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NPHS1 antibody (1019-1241) (STJ11104633)

SKU:
STJ11104633

$174.83 - $863.33
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nephrin (1019-1241) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPHS1
Gene ID: 4868
Uniprot ID: NPHN_HUMAN
Immunogen Region: 1019-1241
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1 (NP_004637.1).
Immunogen Sequence: LGDSGLADKGTQLPITTPGL HQPSGEPEDQLPTEPPSGPS GLPLLPVLFALGGLLLLSNA SCVGGVLWQRRLRRLAEGIS EKTEAGSEEDRVRNEYEESQ WTGERDTQSSTVSTTEAEPY YRSLRDFSPQLPPTQEEVSY SRGFTGEDEDMAFPGHLYDE VERTYPPSGAWGPLYDEVQM GPWDLHWPEDTYQDPRGIYD QVAGDLDTLEPDSLPFELRG HLV
Tissue Specificity Specifically expressed in podocytes of kidney glomeruli.
Post Translational Modifications Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1.
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Protein Name Nephrin
Renal Glomerulus-Specific Cell Adhesion Receptor
Database Links Reactome: R-HSA-373753
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Predominantly Located At Podocyte Slit Diaphragm Between Podocyte Foot Processes
Also Associated With Podocyte Apical Plasma Membrane
Alternative Antibody Names Anti-Nephrin antibody
Anti-Renal Glomerulus-Specific Cell Adhesion Receptor antibody
Anti-NPHS1 antibody
Anti-NPHN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance