• Western blot analysis of extracts of various cell lines, using NOX3 antibody (STJ26439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NOX3 antibody (223-395) (STJ26439)

SKU:
STJ26439

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOX3 (223-395) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOX3
Gene ID: 50508
Uniprot ID: NOX3_HUMAN
Immunogen Region: 223-395
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 223-395 of human NOX3 (NP_056533.1).
Immunogen Sequence: GRIVRGQTQDSLSLHNITFC RDRYAEWQTVAQCPVPQFSG KEPSAWKWILGPVVLYACER IIRFWRFQQEVVITKVVSHP SGVLELHMKKRGFKMAPGQY ILVQCPAISSLEWHPFTLTS APQEDFFSVHIRAAGDWTAA LLEAFGAEGQALQEPWSLPR LAVDGPFGTALTD
Function NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.
Protein Name Nadph Oxidase 3
Mitogenic Oxidase 2
Mox-2
Gp91phox Homolog 3
Gp91-3
Database Links Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadph Oxidase 3 antibody
Anti-Mitogenic Oxidase 2 antibody
Anti-Mox-2 antibody
Anti-Gp91phox Homolog 3 antibody
Anti-Gp91-3 antibody
Anti-NOX3 antibody
Anti-MOX2 antibody

Information sourced from Uniprot.org

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