• Western blot analysis of extracts of various cell lines, using DHODH antibody (STJ115259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human colon using DHODH antibody (STJ115259) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using DHODH antibody (STJ115259) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using DHODH antibody (STJ115259) at dilution of 1:200 (40x lens).

Anti-DHODH antibody (116-395) (STJ115259)

SKU:
STJ115259

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DHODH (116-395) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DHODH
Gene ID: 1723
Uniprot ID: PYRD_HUMAN
Immunogen Region: 116-395
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2).
Immunogen Sequence: EIGSVTPKPQEGNPRPRVFR LPEDQAVINRYGFNSHGLSV VEHRLRARQQKQAKLTEDGL PLGVNLGKNKTSVDAAEDYA EGVRVLGPLADYLVVNVSSP NTAGLRSLQGKAELRRLLTK VLQERDGLRRVHRPAVLVKI APDLTSQDKEDIASVVKELG IDGLIVTNTTVSRPAGLQGA LRSETGGLSGKPLRDLSTQT IREMYALTQGRVPIIGVGGV SSGQDALEKIRAGASLVQL
Post Translational Modifications The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
Function Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.
Protein Name Dihydroorotate Dehydrogenase
Quinone - Mitochondrial
Dhodehase
Dihydroorotate Oxidase
Database Links Reactome: R-HSA-500753
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Dihydroorotate Dehydrogenase antibody
Anti-Quinone - Mitochondrial antibody
Anti-Dhodehase antibody
Anti-Dihydroorotate Oxidase antibody
Anti-DHODH antibody

Information sourced from Uniprot.org

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