Anti-NOTCH2/3 antibody [59R1] {PE} (STJA0031452)

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STJA0031452
£310.50 - £472.50
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Host: Rat
Applications: ELISA/FC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rat monoclonal antibody anti-NOTCH2/3 is suitable for use in ELISA and Flow Cytometry research applications.
Clonality : Monoclonal
Clone ID : 59R1
Conjugation: PE
Isotype: IgG1k
Formulation: 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
Purification: Purified
Concentration: 0.5 mg/mL
Storage Instruction: Store at 4°C for 12 months. Protect from light. Do not freeze.
Gene Symbol: Notch2
Gene ID: 18129
Uniprot ID: NOTC2_MOUSE
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Hydroxylated by HIF1AN. Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. Phosphorylated by GSK3. GSK3-mediated phosphorylation is necessary for NOTCH2 recognition by FBXW7, ubiquitination and degradation via the ubiquitin proteasome pathway.
Function Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells.
Protein Name Neurogenic Locus Notch Homolog Protein 2
Notch 2
Motch B Cleaved Into - Notch 2 Extracellular Truncation - Notch 2 Intracellular Domain
Cellular Localisation Notch 2 Extracellular Truncation: Cell Membrane
Single-Pass Type I Membrane Protein
Notch 2 Intracellular Domain: Nucleus
Cytoplasm
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Retained At The Cytoplasm By Tcim
Alternative Antibody Names Anti-Neurogenic Locus Notch Homolog Protein 2 antibody
Anti-Notch 2 antibody
Anti-Motch B Cleaved Into - Notch 2 Extracellular Truncation - Notch 2 Intracellular Domain antibody
Anti-Notch2 antibody

Information sourced from Uniprot.org