| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nucleolar and coiled-body phosphoprotein 1 (620-700) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:40000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOLC1 |
| Gene ID: |
9221 |
| Uniprot ID: |
NOLC1_HUMAN |
| Immunogen Region: |
620-700 |
| Specificity: |
Nopp140 Polyclonal Antibody detects endogenous levels of Nopp140 protein. |
| Immunogen: |
Synthesized peptide derived from Nopp140. at AA range: 620-700 |
| Post Translational Modifications | Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell. Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Pyrophosphorylated by 5-diphosphoinositol pentakisphosphate (5-IP7). Serine pyrophosphorylation is achieved by Mg(2+)-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue. |
| Function | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. |
| Protein Name | Nucleolar And Coiled-Body Phosphoprotein 1140 Kda Nucleolar PhosphoproteinNopp140Hepatitis C Virus Ns5a-Transactivated Protein 13Hcv Ns5a-Transactivated Protein 13Nucleolar 130 Kda ProteinNucleolar Phosphoprotein P130 |
| Cellular Localisation | NucleusNucleolusCytoplasmShuttles Between The Nucleolus And The CytoplasmAt Telophase It Begins To Assemble Into Granular-Like Pre-Nucleolar Bodies Which Are Subsequently Relocated To Nucleoli At The Early G1-Phase |
| Alternative Antibody Names | Anti-Nucleolar And Coiled-Body Phosphoprotein 1 antibodyAnti-140 Kda Nucleolar Phosphoprotein antibodyAnti-Nopp140 antibodyAnti-Hepatitis C Virus Ns5a-Transactivated Protein 13 antibodyAnti-Hcv Ns5a-Transactivated Protein 13 antibodyAnti-Nucleolar 130 Kda Protein antibodyAnti-Nucleolar Phosphoprotein P130 antibodyAnti-NOLC1 antibodyAnti-KIAA0035 antibodyAnti-NS5ATP13 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
