• Western blot analysis of various lysates using NME7 Rabbit polyclonal antibody (STJ117082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NME7 antibody (1-376) (STJ117082)

SKU:
STJ117082

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NME7
Gene ID: 29922
Uniprot ID: NDK7_HUMAN
Immunogen Region: 1-376
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-376 of human NME7 (NP_037462.1).
Immunogen Sequence: MNHSERFVFIAEWYDPNASL LRRYELLFYPGDGSVEMHDV KNHRTFLKRTKYDNLHLEDL FIGNKVNVFSRQLVLIDYGD QYTARQLGSRKEKTLALIKP DAISKAGEIIEIINKAGFTI TKLKMMMLSRKEALDFHVDH QSRPFFNELIQFITTGPIIA MEILRDDAICEWKRLLGPAN SGVARTDASESIRALFGTDG IRNAAHGPDSFASAAREMEL FFPSSGGCGPANTAKFTNC
Tissue Specificity Expressed in airway epithelial cells.
Post Translational Modifications Undergoes autophosphorylation.
Function Possesses an intrinsic kinase activity. Displays 3'-5' exonuclease activity with a preference for single-stranded DNA. Does not seem to have nucleoside diphosphate kinase activity. Functional component of the gamma-tubulin ring complex, implicated in the regulation of the microtubule-nucleating activity of the gamma-tubulin ring complex in centrosomes, in a kinase activity-dependent manner. Part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.
Protein Name Nucleoside Diphosphate Kinase Homolog 7
Ndk 7
Ndp Kinase Homolog 7
3'-5' Exonuclease Nme7
Protein Kinase Nme7
Nm23-H7
Database Links Reactome: R-HSA-380270
Reactome: R-HSA-380320
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Spindle
Cilium Axoneme
Flagellum Axoneme
Localizes To Centrosomes Through Its Assembly Into Gamma-Tubulin Ring Complex
The Centrosomal Content Of Nme7 Varies During The Cell Cycle
Being Highest In Mitosis And Lowest In Early G1
Alternative Antibody Names Anti-Nucleoside Diphosphate Kinase Homolog 7 antibody
Anti-Ndk 7 antibody
Anti-Ndp Kinase Homolog 7 antibody
Anti-3'-5' Exonuclease Nme7 antibody
Anti-Protein Kinase Nme7 antibody
Anti-Nm23-H7 antibody
Anti-NME7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance