• Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-NHEJ1 antibody (1-299) (STJ11103937)

SKU:
STJ11103937

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Non-Homologous End-Joining Factor 1 (1-299) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NHEJ1
Gene ID: 79840
Uniprot ID: NHEJ1_HUMAN
Immunogen Region: 1-299
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).
Immunogen Sequence: MEELEQGLLMQPWAWLQLAE NSLLAKVFITKQGYALLVSD LQQVWHEQVDTSVVSQRAKE LNKRLTAPPAAFLCHLDNLL RPLLKDAAHPSEATFSCDCV ADALILRVRSELSGLPFYWN FHCMLASPSLVSQHLIRPLM GMSLALQCQVRELATLLHMK DLEIQDYQESGATLIRDRLK TEPFEENSFLEQFMIEKLPE ACSIGDGKPFVMNLQDLYMA VTTQEVQVGQKHQGAGDPH
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by PRKDC at the C-terminus in response to DNA damage. Phosphorylations by PRKDC at the C-terminus of XRCC4 and NHEJ1/XLF are highly redundant and regulate ability of the XRCC4-NHEJ1/XLF subcomplex to bridge DNA. Phosphorylation does not prevent interaction with XRCC4 but disrupts ability to bridge DNA and promotes detachment from DNA.
Function DNA repair protein involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. Associates with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. Binds DNA in a length-dependent manner.
Protein Name Non-Homologous End-Joining Factor 1
Protein Cernunnos
Xrcc4-Like Factor
Database Links Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Chromosome
Localizes To Site Of Double-Strand Breaks
Recruitment Is Dependent On Xrcc5-Xrcc6 (Ku) Heterodimer
Alternative Antibody Names Anti-Non-Homologous End-Joining Factor 1 antibody
Anti-Protein Cernunnos antibody
Anti-Xrcc4-Like Factor antibody
Anti-NHEJ1 antibody
Anti-XLF antibody

Information sourced from Uniprot.org

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