• Western blot analysis of extracts of various cell lines, using NFRKB antibody (STJ112163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NFRKB antibody (1-340) (STJ112163)

SKU:
STJ112163

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFRKB (1-340) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFRKB
Gene ID: 4798
Uniprot ID: NFRKB_HUMAN
Immunogen Region: 1-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human NFRKB (NP_001137307.1).
Immunogen Sequence: MDSLDHMLTDPLELGPCGDG HGTRIMEDCLLGGTRVSLPE DLLEDPEIFFDVVSLSTWQE VLSDSQREHLQQFLPQFPED SAEQQNELILALFSGENFRF GNPLHIAQKLFRDGHFNPEV VKYRQLCFKSQYKRYLNSQQ QYFHRLLKQILASRSDLLEM ARRSGPALPFRQKRPSPSRT PEEREWRTQQRYLKVLREVK EECGDTALSSDEEDLSSWLP SSPARSPSPAVPLRVVPTL
Tissue Specificity Expressed in thymus, brain, testes, spleen and liver.
Function Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'. Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Modulates the deubiquitinase activity of UCHL5 in the INO80 complex.
Protein Name Nuclear Factor Related To Kappa-B-Binding Protein
Dna-Binding Protein R Kappa-B
Ino80 Complex Subunit G
Database Links Reactome: R-HSA-5689603
Reactome: R-HSA-5696394
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor Related To Kappa-B-Binding Protein antibody
Anti-Dna-Binding Protein R Kappa-B antibody
Anti-Ino80 Complex Subunit G antibody
Anti-NFRKB antibody
Anti-INO80G antibody

Information sourced from Uniprot.org

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