• Western blot analysis of extracts of various cell lines, using NEUROD1 antibody (STJ24745) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NEUROD1 antibody (160-356) (STJ24745)

SKU:
STJ24745

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEUROD1 (160-356) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEUROD1
Gene ID: 4760
Uniprot ID: NDF1_HUMAN
Immunogen Region: 160-356
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-356 of human NEUROD1 (NP_002491.2).
Immunogen Sequence: GKSPDLVSFVQTLCKGLSQP TTNLVAGCLQLNPRTFLPEQ NQDMPPHLPTASASFPVHPY SYQSPGLPSPPYGTMDSSHV FHVKPPPHAYSAALEPFFES PLTDCTSPSFDGPLSPPLSI NGNFSFKHEPSAEFEKNYAF TMHYPAATLAGAQSHGSIFS GTAAPRCEIPIDNIMSFDSH SHHERVMSAQLNAIFHD
Post Translational Modifications Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation.which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335.which promotes dendritic growth. Phosphorylated by MAPK1.phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells.
Function Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.
Protein Name Neurogenic Differentiation Factor 1
Neurod
Neurod1
Class A Basic Helix-Loop-Helix Protein 3
Bhlha3
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-210746
Cellular Localisation Cytoplasm
Nucleus
In Pancreatic Islet Cells
Shuttles To The Nucleus In Response To Glucose Stimulation
Colocalizes With Nr0b2 In The Nucleus
Alternative Antibody Names Anti-Neurogenic Differentiation Factor 1 antibody
Anti-Neurod antibody
Anti-Neurod1 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 3 antibody
Anti-Bhlha3 antibody
Anti-NEUROD1 antibody
Anti-BHLHA3 antibody
Anti-NEUROD antibody

Information sourced from Uniprot.org

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