• Western blot analysis of extracts of various cell lines, using NDUFS5 antibody (STJ24726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using NDUFS5 rabbit polyclonal antibody (STJ24726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NDUFS5 antibody (1-106) (STJ24726)

SKU:
STJ24726

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS5 (1-106) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS5
Gene ID: 4725
Uniprot ID: NDUS5_HUMAN
Immunogen Region: 1-106
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human NDUFS5 (NP_004543.1).
Immunogen Sequence: MPFLDIQKRFGLNIDRWLTI QSGEQPYKMAGRCHAFEKEW IECAHGIGYTRAEKECKIEY DDFVECLLRQKTMRRAGTIR KQRDKLIKEGKYTPPPHHIG KGEPRP
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5
Complex I-15 Kda
Ci-15 Kda
Nadh-Ubiquinone Oxidoreductase 15 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5 antibody
Anti-Complex I-15 Kda antibody
Anti-Ci-15 Kda antibody
Anti-Nadh-Ubiquinone Oxidoreductase 15 Kda Subunit antibody
Anti-NDUFS5 antibody

Information sourced from Uniprot.org

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