• Western blot analysis of various lysates using NDUFAF1 Rabbit polyclonal antibody (STJ118280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 35s.

Anti-NDUFAF1 antibody (1-327) (STJ118280)

SKU:
STJ118280

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFAF1
Gene ID: 51103
Uniprot ID: CIA30_HUMAN
Immunogen Region: 1-327
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2).
Immunogen Sequence: MALVHKLLRGTYFLRKFSKP TSALYPFLGIRFAEYSSSLQ KPVASPGKASSQRKTEGDLQ GDHQKEVALDITSSEEKPDV SFDKAIRDEAIYHFRLLKDE IVDHWRGPEGHPLHEVLLEQ AKVVWQFRGKEDLDKWTVTS DKTIGGRSEVFLKMGKNNQS ALLYGTLSSEAPQDGESTRS GYCAMISRIPRGAFERKMSY DWSQFNTLYLRVRGDGRPWM VNIKEDTDFFQRTNQMYSY
Tissue Specificity Ubiquitous.
Function As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Protein Name Complex I Intermediate-Associated Protein 30 - Mitochondrial
Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 1
Database Links Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Peripherally Associated With The Matrix Face Of The Mitochondrial Inner Membrane
Alternative Antibody Names Anti-Complex I Intermediate-Associated Protein 30 - Mitochondrial antibody
Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 1 antibody
Anti-NDUFAF1 antibody
Anti-CIA30 antibody
Anti-CGI-65 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance