Western blot analysis of various lysates using NCEH1 Rabbit polyclonal antibody (STJ119782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NCEH1 antibody (90-440) (STJ119782)

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STJ119782
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCEH1
Gene ID: 57552
Uniprot ID: NCEH1_HUMAN
Immunogen Region: 90-440
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 90-440 of human NCEH1 (NP_065843.3).
Immunogen Sequence: HLLALNFIIVSFGKKSAWSS AQVKVTDTDFDGVEVRVFEG PPKPEEPLKRSVVYIHGGGW ALASAKIRYYDELCTAMAEE LNAVIVSIEYRLVPKVYFPE QIHDVVRATKYFLKPEVLQK YMVDPGRICISGDSAGGNLA AALGQQFTQDASLKNKLKLQ ALIYPVLQALDFNTPSYQQN VNTPILPRYVMVKYWVDYFK GNYDFVQAMIVNNHTSLDVE EAAAVRARLNWTSLLPASF
Tissue Specificity Expressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines.
Post Translational Modifications N-glycosylated.
Function Hydrolyzes 2-acetyl monoalkylglycerol ether (1-O-alkyl-2-acetyl-sn-glycerol), the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for the hydrolysis of cholesterol esters (such as cholesteryl (9Z-octadecenoate)) in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
Protein Name Neutral Cholesterol Ester Hydrolase 1
Nceh
Acetylalkylglycerol Acetylhydrolase
2-Acetyl Mage Hydrolase
Arylacetamide Deacetylase-Like 1
Database Links Reactome: R-HSA-8964038
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Microsome
Alternative Antibody Names Anti-Neutral Cholesterol Ester Hydrolase 1 antibody
Anti-Nceh antibody
Anti-Acetylalkylglycerol Acetylhydrolase antibody
Anti-2-Acetyl Mage Hydrolase antibody
Anti-Arylacetamide Deacetylase-Like 1 antibody
Anti-NCEH1 antibody
Anti-AADACL1 antibody
Anti-KIAA1363 antibody

Information sourced from Uniprot.org

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