• Immunofluorescence analysis of mouse heart using MYL2 Rabbit polyclonal antibody (STJ116121) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat heart using MYL2 Rabbit polyclonal antibody (STJ116121) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MYL2 antibody (STJ116121) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MYL2 antibody (1-166) (STJ116121)

SKU:
STJ116121

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYL2 (1-166) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYL2
Gene ID: 4633
Uniprot ID: MLRV_HUMAN
Immunogen Region: 1-166
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).
Immunogen Sequence: MAPKKAKKRAGGANSNVFSM FEQTQIQEFKEAFTIMDQNR DGFIDKNDLRDTFAALGRVN VKNEEIDEMIKEAPGPINFT VFLTMFGEKLKGADPEETIL NAFKVFDPEGKGVLKADYVR EMLTTQAERFSKEEVDQMFA AFPPDVTGNLDYKNLVHIIT HGEEKD
Tissue Specificity Highly expressed in type I muscle fibers.
Post Translational Modifications N-terminus is methylated by METTL11A/NTM1. Phosphorylated by MYLK3 and MYLK2.promotes cardiac muscle contraction and function. Dephosphorylated by PPP1CB complexed to PPP1R12B. The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation).regulates cardiac torsion and workload distribution.
Function Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion.as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly.
Protein Name Myosin Regulatory Light Chain 2 - Ventricular/Cardiac Muscle Isoform
Mlc-2
Mlc-2v
Cardiac Myosin Light Chain 2
Myosin Light Chain 2 - Slow Skeletal/Ventricular Muscle Isoform
Mlc-2s/V
Ventricular Myosin Light Chain 2
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
A Band
Alternative Antibody Names Anti-Myosin Regulatory Light Chain 2 - Ventricular/Cardiac Muscle Isoform antibody
Anti-Mlc-2 antibody
Anti-Mlc-2v antibody
Anti-Cardiac Myosin Light Chain 2 antibody
Anti-Myosin Light Chain 2 - Slow Skeletal/Ventricular Muscle Isoform antibody
Anti-Mlc-2s/V antibody
Anti-Ventricular Myosin Light Chain 2 antibody
Anti-MYL2 antibody
Anti-MLC2 antibody

Information sourced from Uniprot.org

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