• Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, CD20 monoclonal antibody (2F4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1, CD20 monoclonal antibody (2F4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-MS4A1 antibody [2F4] (STJ96950)

SKU:
STJ96950

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-B-lymphocyte antigen CD20 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 2F4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC 1:200
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MS4A1
Gene ID: 931
Uniprot ID: CD20_HUMAN
Specificity: The antibody detects endogenous CD20 proteins.
Immunogen: Synthetic Peptide of CD20
Post Translational Modifications Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
Function B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
Protein Name B-Lymphocyte Antigen Cd20
B-Lymphocyte Surface Antigen B1
Bp35
Leukocyte Surface Antigen Leu-16
Membrane-Spanning 4-Domains Subfamily A Member 1
Cd Antigen Cd20
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lipid-Anchor
Constitutively Associated With Membrane Rafts
Alternative Antibody Names Anti-B-Lymphocyte Antigen Cd20 antibody
Anti-B-Lymphocyte Surface Antigen B1 antibody
Anti-Bp35 antibody
Anti-Leukocyte Surface Antigen Leu-16 antibody
Anti-Membrane-Spanning 4-Domains Subfamily A Member 1 antibody
Anti-Cd Antigen Cd20 antibody
Anti-MS4A1 antibody
Anti-CD20 antibody

Information sourced from Uniprot.org

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