| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MRAS |
| Gene ID: |
22808 |
| Uniprot ID: |
RASM_HUMAN |
| Immunogen Region: |
79-208 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 79-208 of human MRAS (NP_036351.3). |
| Immunogen Sequence: |
EQYMRTGDGFLIVYSVTDKA SFEHVDRFHQLILRVKDRES FPMILVANKVDLMHLRKITR EQGKEMATKHNIPYIETSAK DPPLNVDKAFHDLVRVIRQQ IPEKSQKKKKKTKWRGDRAT GTHKLQCVIL |
| Tissue Specificity | Expression highly restricted to the brain and heart. |
| Function | Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation. The formation of the SMP complex only occurs when MRAS is GTP-bound. The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities. |
| Protein Name | Ras-Related Protein M-RasRas-Related Protein R-Ras3 |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic Side |
| Alternative Antibody Names | Anti-Ras-Related Protein M-Ras antibodyAnti-Ras-Related Protein R-Ras3 antibodyAnti-MRAS antibodyAnti-RRAS3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and FADD knockout (KO) HeLa cells, using [KO Validated] FADD Rabbit polyclonal antibody (STJ11100017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89090/357549/STJ11100017_1__19285.1713121669.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FADD knockout (KO) HeLa cells, using [KO Validated] FADD Rabbit polyclonal antibody (STJ11100017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")