• Western blot analysis of MPHOSPH9 Antibody. The lane on the right is blocked with the MPHOSPH9 peptide.
  • Western blot analysis of the lysates from COLO205 cells using MPHOSPH9 antibody.
  • Western blot analysis of various cells using MPP9 Polyclonal Antibody

Anti-MPHOSPH9 antibody (539-588) (STJ94189)

SKU:
STJ94189

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-M-phase phosphoprotein 9 (539-588) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MPHOSPH9
Gene ID: 10198
Uniprot ID: MPP9_HUMAN
Immunogen Region: 539-588
Specificity: MPP9 Polyclonal Antibody detects endogenous levels of MPP9 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MPHOSPH9. AA range:539-588
Post Translational Modifications TTBK2-mediated phosphorylation at Ser-781 and Ser-788, promotes its ubiquitination at Lys-784 leading to proteasomal degradation, loss of MPHOSPH9 facilitates the removal of the CP110-CEP97 complex from the mother centrioles, promoting the initiation of ciliogenesis. Phosphorylated in M (mitotic) phase. Ubiquitinated at Lys-784, leading to proteasomal degradation.
Function Negatively regulates cilia formation by recruiting the CP110-CEP97 complex (a negative regulator of ciliogenesis) at the distal end of the mother centriole in ciliary cells. At the beginning of cilia formation, MPHOSPH9 undergoes TTBK2-mediated phosphorylation and degradation via the ubiquitin-proteasome system and removes itself and the CP110-CEP97 complex from the distal end of the mother centriole, which subsequently promotes cilia formation.
Protein Name M-Phase Phosphoprotein 9
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Golgi Apparatus Membrane
Peripheral Membrane Protein
Localizes To The Distal And Proximal End Of Centriole Pairs In Duplicated Centrosomes
In Ciliated Cells
Localizes To The Distal And Proximal End Of Daughter Centriole And Proximal Of The Mother Centriole But Not In The Distal End Of The Mother Centriole
Recruited By Kif24 To The Distal End Of Mother Centriole Where It Forms A Ring-Like Structure
Alternative Antibody Names Anti-M-Phase Phosphoprotein 9 antibody
Anti-MPHOSPH9 antibody
Anti-MPP9 antibody

Information sourced from Uniprot.org

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