| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MLLT3 (1-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MLLT3 |
| Gene ID: |
4300 |
| Uniprot ID: |
AF9_HUMAN |
| Immunogen Region: |
1-150 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MLLT3/AF9 (NP_004520.2). |
| Immunogen Sequence: |
MASSCAVQVKLELGHRAQVR KKPTVEGFTHDWMVFVRGPE HSNIQHFVEKVVFHLHESFP RPKRVCKDPPYKVEESGYAG FILPIEVYFKNKEEPRKVRF DYDLFLHLEGHPPVNHLRCE KLTFNNPTEDFRRKLLKAGG DPNRSIHTSS |
| Tissue Specificity | Enriched in undifferentiated hematopoietic stem cells in fetal liver, cord blood and bone marrow. |
| Function | Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me). Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than confering, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L. |
| Protein Name | Protein Af-9All1-Fused Gene From Chromosome 9 ProteinMyeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 ProteinYeats Domain-Containing Protein 3 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-674695Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeColocalizes With Acylated Histone H3Colocalizes With Histone H3 Crotonylated At 'Lys-18' (H3k18cr) |
| Alternative Antibody Names | Anti-Protein Af-9 antibodyAnti-All1-Fused Gene From Chromosome 9 Protein antibodyAnti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein antibodyAnti-Yeats Domain-Containing Protein 3 antibodyAnti-MLLT3 antibodyAnti-AF9 antibodyAnti-YEATS3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
