• Western blot analysis of 1) MCF7, 2) Mouse Brain Tissue, 3) Rat Brain Tissue using MICU1 monoclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using MICU1 monoclonal antibody.
  • Immunofluorescence analysis of Human-appendix tissue. 1, MICU1 monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, MICU1 monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, MICU1 monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, MICU1 monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MICU1 antibody [Mix] (STJ97447)

SKU:
STJ97447

£48.00 - £252.00
Current Stock:
Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Calcium uptake protein 1, mitochondrial is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: Mix
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Dilution Range: WB 1:1000-2000
IHC 1:100-200
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MICU1
Gene ID: 10367
Uniprot ID: MICU1_HUMAN
Specificity: The antibody detects endogenous MICU1 protein.
Immunogen: Recombinant Protein of MICU1
Post Translational Modifications Phosphorylation at Ser-122 by AKT1 impairs its maturation and stability. Asymmetric dimethylation at Arg-455 by PRMT1 decreases the calcium sensitivity of MICU1 by promoting interaction with UCP2. Degraded by YME1L1 when not complexed as homodimer or heterodimer. Not degraded when complexed as homodimer or heterodimer.the presence of the interchain disulfide bond protecting MICU1 from degradation by YME1L1.
Function Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains. MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake. At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel. Also required to protect against manganese toxicity by preventing manganese uptake by MCU: mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion. Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360. Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG. Isoform 6: Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle. Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations. This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise.
Protein Name Calcium Uptake Protein 1 - Mitochondrial
Atopy-Related Autoantigen Calc
Ara Calc
Calcium-Binding Atopy-Related Autoantigen 1
Allergen Hom S 4
Database Links Reactome: R-HSA-8949215
Reactome: R-HSA-8949664
Cellular Localisation Mitochondrion Intermembrane Space
Mitochondrion Inner Membrane
Recruited To The Mitochondrial Inner Membrane By Emre/Smdt1
Also Localizes To Mitochondrial Cristae Junctions
Isoform 6: Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Calcium Uptake Protein 1 - Mitochondrial antibody
Anti-Atopy-Related Autoantigen Calc antibody
Anti-Ara Calc antibody
Anti-Calcium-Binding Atopy-Related Autoantigen 1 antibody
Anti-Allergen Hom S 4 antibody
Anti-MICU1 antibody
Anti-CALC antibody
Anti-CBARA1 antibody

Information sourced from Uniprot.org

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