• Western blot analysis of extracts of various cell lines, using METTL4 antibody (STJ111645) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U2OS cells using METTL4 Rabbit polyclonal antibody (STJ111645) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using METTL4 rabbit polyclonal antibody (STJ111645) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH/3T3 cells using METTL4 Rabbit polyclonal antibody (STJ111645) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using METTL4 rabbit polyclonal antibody (STJ111645) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat testis using METTL4 Rabbit polyclonal antibody (STJ111645) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat testis using METTL4 rabbit polyclonal antibody (STJ111645) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using METTL4 Rabbit polyclonal antibody (STJ111645) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using METTL4 rabbit polyclonal antibody (STJ111645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using METTL4 Rabbit polyclonal antibody (STJ111645) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of PC-12 cells using METTL4 rabbit polyclonal antibody (STJ111645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using METTL4 antibody (STJ111645) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U2OS cells using METTL4 rabbit polyclonal antibody (STJ111645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-METTL4 antibody (1-190) (STJ111645)

SKU:
STJ111645

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-METTL4 (1-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: METTL4
Gene ID: 64863
Uniprot ID: METL4_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human METTL4 (NP_073751.3).
Immunogen Sequence: MSVVHQLSAGWLLDHLSFIN KINYQLHQHHEPCCRKKEFT TSVHFESLQMDSVSSSGVCA AFIASDSSTKPENDDGGNYE MFTRKFVFRPELFDVTKPYI TPAVHKECQQSNEKEDLMNG VKKEISISIIGKKRKRCVVF NQGELDAMEYHTKIRELILD GSLQLIQEGLKSGFLYPLFE KQDKGSKPIT
Function N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m) methylation of snRNAs regulates RNA splicing. Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing.
Protein Name N(6-Adenine-Specific Methyltransferase Mettl4
Methyltransferase-Like Protein 4
N(6-Adenine-Specific Dna Methyltransferase Mettl4
Snrna
2'-O-Methyladenosine-N(6--Methyltransferase Mettl4
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Mitochondrion Matrix
Alternative Antibody Names Anti-N(6-Adenine-Specific Methyltransferase Mettl4 antibody
Anti-Methyltransferase-Like Protein 4 antibody
Anti-N(6-Adenine-Specific Dna Methyltransferase Mettl4 antibody
Anti-Snrna antibody
Anti-2'-O-Methyladenosine-N(6--Methyltransferase Mettl4 antibody
Anti-METTL4 antibody

Information sourced from Uniprot.org

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