• Immunoprecipitation of METTL3 from 200 µg extracts of 293F cells was performed using 0. 5 µg of METTL3 Rabbit monoclonal antibody (STJ11101616). Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using METTL3 Rabbit monoclonal antibody (STJ11101616) at a dilution of 1:500.
  • Confocal imaging of HeLa cells using METTL3 Rabbit monoclonal antibody (STJ11101616, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of lysates from HeLa cells, using METTL3 Rabbit monoclonal antibody (STJ11101616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-METTL3 antibody (2-250) [S6MR] (STJ11101616)


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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Short Description: Rabbit monoclonal antibody anti-METTL3 (2-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: METTL3
Gene ID: 56339
Uniprot ID: MTA70_HUMAN
Immunogen Region: 2-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-250 of human METTL3 (Q86U44).
Tissue Specificity Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Post Translational Modifications Sumoylation inhibits the N6-adenosine-methyltransferase activity. Sumoylation does not affect subcellular location or interaction with METTL14. Desumoylated by SENP1.
Function The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-AGGAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop. M6A also regulates circadian regulation of hepatic lipid metabolism. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. Also required for oogenesis. Involved in the response to DNA damage: in response to ultraviolet irradiation, METTL3 rapidly catalyzes the formation of m6A on poly(A) transcripts at DNA damage sites, leading to the recruitment of POLK to DNA damage sites. M6A is also required for T-cell homeostasis and differentiation: m6A methylation of transcripts of SOCS family members (SOCS1, SOCS3 and CISH) in naive T-cells promotes mRNA destabilization and degradation, promoting T-cell differentiation. Inhibits the type I interferon response by mediating m6A methylation of IFNB. M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). Mediates m6A methylation of Xist RNA, thereby participating in random X inactivation: m6A methylation of Xist leads to target YTHDC1 reader on Xist and promote transcription repression activity of Xist. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells. METTL3 mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8. Acts as a positive regulator of mRNA translation independently of the methyltransferase activity: promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate in cancer cell proliferation by promoting mRNA translation. During human coronorivus SARS-CoV-2 infection, adds m6A modifications in SARS-CoV-2 RNA leading to decreased RIGI binding and subsequently dampening the sensing and activation of innate immune responses.
Protein Name N6-Adenosine-Methyltransferase Catalytic Subunit
Methyltransferase-Like Protein 3
N6-Adenosine-Methyltransferase 70 Kda Subunit
Database Links Reactome: R-HSA-72203
Cellular Localisation Nucleus
Nucleus Speckle
Colocalizes With Speckles In Interphase Nuclei
Suggesting That It May Be Associated With Nuclear Pre-Mrna Splicing Components
In Response To Ultraviolet Irradiation
Colocalizes To Dna Damage Sites However
It Probably Does Not Bind Dna But Localizes In The Vicinity Of Dna Damage Sites
Alternative Antibody Names Anti-N6-Adenosine-Methyltransferase Catalytic Subunit antibody
Anti-Methyltransferase-Like Protein 3 antibody
Anti-Hmettl3 antibody
Anti-N6-Adenosine-Methyltransferase 70 Kda Subunit antibody
Anti-Mt-A70 antibody
Anti-METTL3 antibody
Anti-MTA70 antibody

Information sourced from Uniprot.org

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