• Western blot analysis of extracts of various cell lines, using METTL14 antibody (STJ111272) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Anti-METTL14 antibody (1-210) (STJ111272)
  • Immunohistochemistry of paraffin-embedded mouse brain using METTL14 antibody (STJ111272) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Anti-METTL14 antibody (1-210) (STJ111272)
  • Immunohistochemistry of paraffin-embedded Rat spleen using METTL14 antibody (STJ111272) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using METTL14 Rabbit polyclonal antibody (STJ111272) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human mammary cancer using METTL14 antibody (STJ111272) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using METTL14 Rabbit polyclonal antibody (STJ111272) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using METTL14 rabbit polyclonal antibody (STJ111272) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using METTL14 Rabbit polyclonal antibody (STJ111272) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of PC-12 cells using METTL14 rabbit polyclonal antibody (STJ111272) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using METTL14 rabbit polyclonal antibody (STJ111272) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug METTL14 antibody (STJ111272). Western blot was performed from the immunoprecipitate using METTL14 antibody (STJ111272) at a dilition of 1:1000.

Anti-METTL14 antibody (1-210) (STJ111272)

SKU:
STJ111272

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: METTL14
Gene ID: 57721
Uniprot ID: MET14_HUMAN
Immunogen Region: 1-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1).
Immunogen Sequence: MDSRLQEIRERQKLRRQLLA QQLGAESADSIGAVLNSKDE QREIAETRETCRASYDTSAP NAKRKYLDEGETDEDKMEEY KDELEMQQDEENLPYEEEIY KDSSTFLKGTQSLNPHNDYC QHFVDTGHRPQNFIRDVGLA DRFEEYPKLRELIRLKDELI AKSNTPPMYLQADIEAFDIR ELTPKFDVILLEPPLEEYYR ETGITANEKC
Function The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-AGGAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells.
Protein Name N(6-Adenosine-Methyltransferase Non-Catalytic Subunit Mettl14
Methyltransferase-Like Protein 14
Hmettl14
Database Links Reactome: R-HSA-72203
Cellular Localisation Nucleus
Alternative Antibody Names Anti-N(6-Adenosine-Methyltransferase Non-Catalytic Subunit Mettl14 antibody
Anti-Methyltransferase-Like Protein 14 antibody
Anti-Hmettl14 antibody
Anti-METTL14 antibody
Anti-KIAA1627 antibody

Information sourced from Uniprot.org

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