• Immunofluorescence analysis of A549 cells using METTL13 antibody (STJ114456). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of MCF7 cells, using METTL13 antibody (STJ114456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-METTL13 antibody (430-699) (STJ114456)

SKU:
STJ114456

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-METTL13 (430-699) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: METTL13
Gene ID: 51603
Uniprot ID: EFNMT_HUMAN
Immunogen Region: 430-699
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human METTL13 (NP_057019.3).
Immunogen Sequence: LKDVSHKAQKKRKKDRKKQR PADAEDLPAAPGQSIDKSYL CCEHHKAMIAGLALLRNPEL LLEIPLALLVVGLGGGSLPL FVHDHFPKSCIDAVEIDPSM LEVATQWFGFSQSDRMKVHI ADGLDYIASLAGGGEARPCY DVIMFDVDSKDPTLGMSCPP PAFVEQSFLQKVKSILTPEG VFILNLVCRDLGLKDSVLAG LKAVFPLLYVRRIEGEVNEI LFCQLHPEQKLATPELLET
Function Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation. Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2'. Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55'. Has no activity towards core histones H2A, H2B, H3 and H4. Negatively regulates cell proliferation at G1/S transition via transcriptional suppression of cell cycle regulatory genes such as CDK4 and CDK6.
Protein Name Eef1a Lysine And N-Terminal Methyltransferase
Eef1a-Knmt
Methyltransferase-Like Protein 13 Includes - Eef1a Lysine Methyltransferase - Eef1a N-Terminal Methyltransferase
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Alternative Antibody Names Anti-Eef1a Lysine And N-Terminal Methyltransferase antibody
Anti-Eef1a-Knmt antibody
Anti-Methyltransferase-Like Protein 13 Includes - Eef1a Lysine Methyltransferase - Eef1a N-Terminal Methyltransferase antibody
Anti-METTL13 antibody
Anti-EEF1AKNMT antibody
Anti-FEAT antibody
Anti-KIAA0859 antibody
Anti-CGI-01 antibody

Information sourced from Uniprot.org

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