• Immunohistochemistry analysis of paraffin-embedded human lung cancer using MET Rabbit polyclonal antibody (STJ119492) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MET antibody (STJ119492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MET antibody (518-840) (STJ119492)

SKU:
STJ119492

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MET (518-840) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MET
Gene ID: 4233
Uniprot ID: MET_HUMAN
Immunogen Region: 518-840
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 518-840 of human MET (NP_000236.2).
Immunogen Sequence: LGCRHFQSCSQCLSAPPFVQ CGWCHDKCVRSEECLSGTWT QQICLPAIYKVFPNSAPLEG GTRLTICGWDFGFRRNNKFD LKKTRVLLGNESCTLTLSES TMNTLKCTVGPAMNKHFNMS IIISNGHGTTQYSTFSYVDP VITSISPKYGPMAGGTLLTL TGNYLNSGNSRHISIGGKTC TLKSVSNSILECYTPAQTIS TEFAVKLKIDLANRETSIFS YREDPIVYEIHPTKSFISG
Tissue Specificity Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level).
Post Translational Modifications Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2. Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors. O-mannosylation of IPT/TIG domains by TMEM260 is required for protein maturation. O-mannosylated residues are composed of single mannose glycans that are not elongated or modified. (Microbial infection) Tyrosine phosphorylation is stimulated by L.monocytogenes InlB. Tyrosine phosphorylation is maximal 10-20 minutes after treatment with InlB and disappears by 60 minutes. The phosphorylated residues were not identified.
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis. (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
Protein Name Hepatocyte Growth Factor Receptor
Hgf Receptor
Hgf/Sf Receptor
Proto-Oncogene C-Met
Scatter Factor Receptor
Sf Receptor
Tyrosine-Protein Kinase Met
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-416550
Reactome: R-HSA-5673001
Reactome: R-HSA-6806942
Reactome: R-HSA-6807004
Reactome: R-HSA-6811558
Reactome: R-HSA-8851805
Reactome: R-HSA-8851907
Reactome: R-HSA-8865999
Reactome: R-HSA-8874081
Reactome: R-HSA-8875360
Reactome: R-HSA-8875513
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-8875791
Reactome: R-HSA-9022699
Reactome: R-HSA-9734091
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Secreted
Alternative Antibody Names Anti-Hepatocyte Growth Factor Receptor antibody
Anti-Hgf Receptor antibody
Anti-Hgf/Sf Receptor antibody
Anti-Proto-Oncogene C-Met antibody
Anti-Scatter Factor Receptor antibody
Anti-Sf Receptor antibody
Anti-Tyrosine-Protein Kinase Met antibody
Anti-MET antibody

Information sourced from Uniprot.org

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