• Western blot analysis of extracts of mouse brain, using MED30 rabbit polyclonal antibody (STJ115101) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MED30 antibody (1-178) (STJ115101)

SKU:
STJ115101

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MED30 (1-178) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MED30
Gene ID: 90390
Uniprot ID: MED30_HUMAN
Immunogen Region: 1-178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human MED30 (NP_542382.1).
Immunogen Sequence: MSTPPLAASGMAPGPFAGPQ AQQAAREVNTASLCRIGQET VQDIVYRTMEIFQLLRNMQL PNGVTYHTGTYQDRLTKLQD NLRQLSVLFRKLRLVYDKCN ENCGGMDPIPVEQLIPYVEE DGSKNDDRAGPPRFASEERR EIAEVNKKLKQKNQQLKQIM DQLRNLIWDINAMLAMRN
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Protein Name Mediator Of Rna Polymerase Ii Transcription Subunit 30
Mediator Complex Subunit 30
Trap/Mediator Complex Component Trap25
Thyroid Hormone Receptor-Associated Protein 6
Thyroid Hormone Receptor-Associated Protein Complex 25 Kda Component
Trap25
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-212436
Reactome: R-HSA-381340
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 30 antibody
Anti-Mediator Complex Subunit 30 antibody
Anti-Trap/Mediator Complex Component Trap25 antibody
Anti-Thyroid Hormone Receptor-Associated Protein 6 antibody
Anti-Thyroid Hormone Receptor-Associated Protein Complex 25 Kda Component antibody
Anti-Trap25 antibody
Anti-MED30 antibody
Anti-THRAP6 antibody
Anti-TRAP25 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance