![Immunohistochemistry analysis of MECP2 in paraffin-embedded mouse brain using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93964/375266/STJ114895_1__58287.1713141990.jpg?c=1 "Immunohistochemistry analysis of MECP2 in paraffin-embedded mouse brain using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of MECP2 in paraffin-embedded human gastric cancer using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93964/375267/STJ114895_2__87079.1713141990.jpg?c=1 "Immunohistochemistry analysis of MECP2 in paraffin-embedded human gastric cancer using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and MECP2 knockout (KO) 293T cells, using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93964/375268/STJ114895_3__71798.1713141991.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MECP2 knockout (KO) 293T cells, using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93964/375269/STJ114895_4__71247.1713141992.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry analysis of MECP2 in paraffin-embedded mouse brain using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/93964/375266/STJ114895_1__58287.1713141990.jpg?c=1 "Immunohistochemistry analysis of MECP2 in paraffin-embedded mouse brain using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MECP2 |
| Gene ID: | 4204 |
| Uniprot ID: | MECP2_HUMAN |
| Immunogen Region: | 1-280 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MECP2 (NP_004983.1). |
| Immunogen Sequence: | MVAGMLGLREEKSEDQDLQG LKDKPLKFKKVKKDKKEEKE GKHEPVQPSAHHSAEPAEAG KAETSEGSGSAPAVPEASAS PKQRRSIIRDRGPMYDDPTL PEGWTRKLKQRKSGRSAGKY DVYLINPQGKAFRSKVELIA YFEKVGDTSLDPNDFDFTVT GRGSPSRREQKPPKKPKSPK APGTGRGRGRPKGSGTTRPK AATSEGVQVKRVLEKSPGKL LVKMPFQTSPGGKAEGGGA |
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Post Translational Modifications | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-HSA-8986944Reactome: R-HSA-9022534Reactome: R-HSA-9022535Reactome: R-HSA-9022537Reactome: R-HSA-9022538Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9022702Reactome: R-HSA-9022707Reactome: R-HSA-9022927Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-MECP2 antibody |
Information sourced from Uniprot.org