• Western blot analysis of extracts of various cell lines, using MDH1 rabbit monoclonal antibody (STJ11102525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using MDH1 Rabbit monoclonal antibody (STJ11102525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MDH1 antibody (235-334) [S5MR] (STJ11102525)

SKU:
STJ11102525

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MDH1 (235-334) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDH1
Gene ID: 4190
Uniprot ID: MDHC_HUMAN
Immunogen Region: 235-334
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 235-334 of human MDH1 (P40925).
Immunogen Sequence: IKARKLSSAMSAAKAICDHV RDIWFGTPEGEFVSMGVISD GNSYGVPDDLLYSFPVVIKN KTWKFVEGLPINDFSREKMD LTAKELTEEKESAFEFLSSA
Post Translational Modifications ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.
Function Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS).
Protein Name Malate Dehydrogenase - Cytoplasmic
Aromatic Alpha-Keto Acid Reductase
Kar
Cytosolic Malate Dehydrogenase
Database Links Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Malate Dehydrogenase - Cytoplasmic antibody
Anti-Aromatic Alpha-Keto Acid Reductase antibody
Anti-Kar antibody
Anti-Cytosolic Malate Dehydrogenase antibody
Anti-MDH1 antibody
Anti-MDHA antibody

Information sourced from Uniprot.org

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