• Immunofluorescence analysis of U-2 OS cells using MDC1 Rabbit polyclonal antibody (STJ114587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MDC1 in paraffin-embedded mouse testis using MDC1 Rabbit polyclonal antibody (STJ114587) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MDC1 in paraffin-embedded human esophageal cancer using MDC1 Rabbit polyclonal antibody (STJ114587) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from mouse testis, using MDC1 Rabbit polyclonal antibody (STJ114587) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MDC1 antibody (1-100) (STJ114587)

SKU:
STJ114587

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MDC1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDC1
Gene ID: 9656
Uniprot ID: MDC1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MDC1 (NP_055456.2).
Immunogen Sequence: MEDTQAIDWDVEEEEETEQS SESLRCNVEPVGRLHIFSGA HGPEKDFPLHLGKNVVGRMP DCSVALPFPSISKQHAEIEI LAWDKAPILRDCGSLNGTQI
Tissue Specificity Highly expressed in testis.
Post Translational Modifications Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain. Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation. Ubiquitinated by RNF4, leading to proteasomal degradation.undergoes 'Lys-48'-linked polyubiquitination.
Function Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Protein Name Mediator Of Dna Damage Checkpoint Protein 1
Nuclear Factor With Brct Domains 1
Database Links Reactome: R-HSA-3108214
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-6796648
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Chromosome
Associated With Chromatin
Relocalizes To Discrete Nuclear Foci Following Dna Damage
This Requires 'Ser-139' Phosphorylation Of H2ax
Colocalizes With Aptx At Sites Of Dna Double-Strand Breaks
Alternative Antibody Names Anti-Mediator Of Dna Damage Checkpoint Protein 1 antibody
Anti-Nuclear Factor With Brct Domains 1 antibody
Anti-MDC1 antibody
Anti-KIAA0170 antibody
Anti-NFBD1 antibody

Information sourced from Uniprot.org

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