• Immunohistochemistry analysis of MBTPS1 in paraffin-embedded human colon carcinoma tissue using MBTPS1 Rabbit polyclonal antibody (STJ11105565) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MBTPS1 in paraffin-embedded human liver cancer tissue using MBTPS1 Rabbit polyclonal antibody (STJ11105565) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Rat lung using MBTPS1 Rabbit polyclonal antibody (STJ11105565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MBTPS1 antibody (950-1052) (STJ11105565)

SKU:
STJ11105565

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Membrane-Bound Transcription Factor Site-1 Protease (950-1052) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MBTPS1
Gene ID: 8720
Uniprot ID: MBTP1_HUMAN
Immunogen Region: 950-1052
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 950-1052 of human MBTPS1 (NP_003782.1).
Immunogen Sequence: QKLLSIDLDKVVLPNFRSNR PQVRPLSPGESGAWDIPGGI MPGRYNQEVGQTIPVFAFLG AMVVLAFFVVQINKAKSRPK RRKPRVKRPQLMQQVHPPKT PSV
Tissue Specificity Widely expressed.
Post Translational Modifications The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates.
Function Serine protease that cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4: known substrates include SREBF1/SREBP1, SREBF2/SREBP2, BDNF, GNPTAB, ATF6, ATF6B and FAM20C. Cleaves substrates after Arg-Ser-Val-Leu (SREBP2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Also mediates the first step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B). Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. Cleaves the propeptide from FAM20C which is required for FAM20C secretion from the Golgi apparatus membrane and for enhancement of FAM20C kinase activity, promoting osteoblast differentiation and biomineralization. Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes. It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation. Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking. In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the first step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression.
Protein Name Membrane-Bound Transcription Factor Site-1 Protease
Endopeptidase S1p
Subtilisin/Kexin-Isozyme 1
Ski-1
Database Links Reactome: R-HSA-1655829
Reactome: R-HSA-381033
Reactome: R-HSA-381426
Reactome: R-HSA-8874177
Reactome: R-HSA-8874211
Reactome: R-HSA-8957275
Reactome: R-HSA-8963889
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus Membrane
May Sort To Other Organelles
Including Lysosomal And/Or Endosomal Compartments
Alternative Antibody Names Anti-Membrane-Bound Transcription Factor Site-1 Protease antibody
Anti-Endopeptidase S1p antibody
Anti-Subtilisin/Kexin-Isozyme 1 antibody
Anti-Ski-1 antibody
Anti-MBTPS1 antibody
Anti-KIAA0091 antibody
Anti-S1P antibody
Anti-SKI1 antibody

Information sourced from Uniprot.org

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