| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Membrane-Bound Transcription Factor Site-1 Protease (950-1052) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MBTPS1 |
| Gene ID: |
8720 |
| Uniprot ID: |
MBTP1_HUMAN |
| Immunogen Region: |
950-1052 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 950-1052 of human MBTPS1 (NP_003782.1). |
| Immunogen Sequence: |
QKLLSIDLDKVVLPNFRSNR PQVRPLSPGESGAWDIPGGI MPGRYNQEVGQTIPVFAFLG AMVVLAFFVVQINKAKSRPK RRKPRVKRPQLMQQVHPPKT PSV |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates. |
| Function | Serine protease that cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4: known substrates include SREBF1/SREBP1, SREBF2/SREBP2, BDNF, GNPTAB, ATF6, ATF6B and FAM20C. Cleaves substrates after Arg-Ser-Val-Leu (SREBP2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Also mediates the first step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B). Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. Cleaves the propeptide from FAM20C which is required for FAM20C secretion from the Golgi apparatus membrane and for enhancement of FAM20C kinase activity, promoting osteoblast differentiation and biomineralization. Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes. It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation. Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking. In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the first step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression. |
| Protein Name | Membrane-Bound Transcription Factor Site-1 ProteaseEndopeptidase S1pSubtilisin/Kexin-Isozyme 1Ski-1 |
| Database Links | Reactome: R-HSA-1655829Reactome: R-HSA-381033Reactome: R-HSA-381426Reactome: R-HSA-8874177Reactome: R-HSA-8874211Reactome: R-HSA-8957275Reactome: R-HSA-8963889 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinGolgi Apparatus MembraneMay Sort To Other OrganellesIncluding Lysosomal And/Or Endosomal Compartments |
| Alternative Antibody Names | Anti-Membrane-Bound Transcription Factor Site-1 Protease antibodyAnti-Endopeptidase S1p antibodyAnti-Subtilisin/Kexin-Isozyme 1 antibodyAnti-Ski-1 antibodyAnti-MBTPS1 antibodyAnti-KIAA0091 antibodyAnti-S1P antibodyAnti-SKI1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
