| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-MARK2 (689-788) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S0MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MARK2 |
| Gene ID: |
2011 |
| Uniprot ID: |
MARK2_HUMAN |
| Immunogen Region: |
689-788 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 689-788 of human MARK2 (Q7KZI7). |
| Immunogen Sequence: |
KPRSLRFTWSMKTTSSMEPN EMMREIRKVLDANSCQSELH EKYMLLCMHGTPGHEDFVQW EMEVCKLPRLSLNGVRFKRI SGTSMAFKNIASKIANELKL |
| Tissue Specificity | High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. |
| Post Translational Modifications | Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm. |
| Function | Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. |
| Protein Name | Serine/Threonine-Protein Kinase Mark2Elkl Motif Kinase 1Emk-1Map/Microtubule Affinity-Regulating Kinase 2Par1 HomologPar1 Homolog BPar-1bPar1b |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmLateral Cell MembraneCytoskeletonCell ProjectionDendritePhosphorylation At Thr-596 By Prkcz/Apkc And Subsequent Interaction With 14-3-3 Protein Ywhaz Promotes Relocation From The Cell Membrane To The Cytoplasm |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Mark2 antibodyAnti-Elkl Motif Kinase 1 antibodyAnti-Emk-1 antibodyAnti-Map/Microtubule Affinity-Regulating Kinase 2 antibodyAnti-Par1 Homolog antibodyAnti-Par1 Homolog B antibodyAnti-Par-1b antibodyAnti-Par1b antibodyAnti-MARK2 antibodyAnti-EMK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
