• Western blot analysis of extracts of various cell lines, using MAP4K5 antibody (STJ110269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts of various cell lines, using MAP4K5 antibody (STJ110269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-MAP4K5 antibody (300-480) (STJ110269)

SKU:
STJ110269

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP4K5 (300-480) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP4K5
Gene ID: 11183
Uniprot ID: M4K5_HUMAN
Immunogen Region: 300-480
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-480 of human MAP4K5 (NP_006566.2).
Immunogen Sequence: HAHYTEADDDDFEPHAIIRH TIRSTNRNARAERTASEINF DKLQFEPPLRKETEARDEMG LSSDPNFMLQWNPFVDGANT GKSTSKRAIPPPLPPKPRIS SYPEDNFPDEEKASTIKHCP DSESRAPQILRRQSSPSCGP VAETSSIGNGDGISKLMSEN TEGSAQAPQLPRKKDKRDFP K
Tissue Specificity Ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate.
Function May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5
Kinase Homologous To Sps1/Ste20
Khs
Mapk/Erk Kinase Kinase Kinase 5
Mek Kinase Kinase 5
Mekkk 5
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 antibody
Anti-Kinase Homologous To Sps1/Ste20 antibody
Anti-Khs antibody
Anti-Mapk/Erk Kinase Kinase Kinase 5 antibody
Anti-Mek Kinase Kinase 5 antibody
Anti-Mekkk 5 antibody
Anti-MAP4K5 antibody

Information sourced from Uniprot.org

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