• Confocal imaging of C6 cells using MEK3 Rabbit monoclonal antibody (STJ11101845, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using MEK3 Rabbit monoclonal antibody (STJ11101845, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using MEK3 Rabbit monoclonal antibody (STJ11101845, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using MEK3 Rabbit monoclonal antibody (STJ11101845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP2K3 antibody (150-347) [S5MR] (STJ11101845)

SKU:
STJ11101845

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MEK3 (150-347) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K3
Gene ID: 5606
Uniprot ID: MP2K3_HUMAN
Immunogen Region: 150-347
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-347 of human MEK3 (P46734).
Immunogen Sequence: FYRKVLDKNMTIPEDILGEI AVSIVRALEHLHSKLSVIHR DVKPSNVLINKEGHVKMCDF GISGYLVDSVAKTMDAGCKP YMAPERINPELNQKGYNVKS DVWSLGITMIEMAILRFPYE SWGTPFQQLKQVVEEPSPQL PADRFSPEFVDFTAQCLRKN PAERMSYLELMEHPFFTLHK TKKTDIAAFVKEILGEDS
Tissue Specificity Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Post Translational Modifications Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 3
Map Kinase Kinase 3
Mapkk 3
Mapk/Erk Kinase 3
Mek 3
Stress-Activated Protein Kinase Kinase 2
Sapk Kinase 2
Sapkk-2
Sapkk2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-450302
Reactome: R-HSA-5210891 P46734-3
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 3 antibody
Anti-Map Kinase Kinase 3 antibody
Anti-Mapkk 3 antibody
Anti-Mapk/Erk Kinase 3 antibody
Anti-Mek 3 antibody
Anti-Stress-Activated Protein Kinase Kinase 2 antibody
Anti-Sapk Kinase 2 antibody
Anti-Sapkk-2 antibody
Anti-Sapkk2 antibody
Anti-MAP2K3 antibody
Anti-MEK3 antibody
Anti-MKK3 antibody
Anti-PRKMK3 antibody
Anti-SKK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance