| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MAG (20-220) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAG |
| Gene ID: |
4099 |
| Uniprot ID: |
MAG_HUMAN |
| Immunogen Region: |
20-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human MAG (P20916). |
| Immunogen Sequence: |
GHWGAWMPSSISAFEGTCVS IPCRFDFPDELRPAVVHGVW YFNSPYPKNYPPVVFKSRTQ VVHESFQGRSRLLGDLGLRN CTLLLSNVSPELGGKYYFRG DLGGYNQYTFSEHSVLDIVN TPNIVVPPEVVAGTEVEVSC MVPDNCPELRPELSWLGHEG LGEPAVLGRLREDEGTWVQV SLLHFVPTREANGHRLGCQA S |
| Tissue Specificity | Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures. Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths. Detected in compact myelin (at protein level). Both isoform 1 and isoform 2 are detected in the central and peripheral nervous system. |
| Post Translational Modifications | N-glycosylated. Phosphorylated on tyrosine residues. Ubiquitinated, leading to proteasomal degradation. |
| Function | Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury.protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults.this probably depends on binding to gangliosides on the axon cell membrane. Negative regulator of neurite outgrowth.in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth. Inhibits axon outgrowth by binding to RTN4R. Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b. |
| Protein Name | Myelin-Associated GlycoproteinSiglec-4a |
| Database Links | Reactome: R-HSA-193634Reactome: R-HSA-210991Reactome: R-HSA-9619665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Names | Anti-Myelin-Associated Glycoprotein antibodyAnti-Siglec-4a antibodyAnti-MAG antibodyAnti-GMA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
