• Western blot analysis of extracts of various cell lines, using LTA4H antibody (STJ115957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LTA4H antibody (312-611) (STJ115957)

SKU:
STJ115957

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LTA4H (312-611) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LTA4H
Gene ID: 4048
Uniprot ID: LKHA4_HUMAN
Immunogen Region: 312-611
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 312-611 of human LTA4H (NP_000886.1).
Immunogen Sequence: WDHFWLNEGHTVYLERHICG RLFGEKFRHFNALGGWGELQ NSVKTFGETHPFTKLVVDLT DIDPDVAYSSVPYEKGFALL FYLEQLLGGPEIFLGFLKAY VEKFSYKSITTDDWKDFLYS YFKDKVDVLNQVDWNAWLYS PGLPPIKPNYDMTLTNACIA LSQRWITAKEDDLNSFNATD LKDLSSHQLNEFLAQTLQRA PLPLGHIKRMQEVYNFNAIN NSEIRFRWLRLCIQSKWED
Tissue Specificity Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
Post Translational Modifications Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity.
Function Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions.
Protein Name Leukotriene A-4 Hydrolase
Lta-4 Hydrolase
Leukotriene A(4 Hydrolase
Tripeptide Aminopeptidase Lta4h
Database Links Reactome: R-HSA-2142691
Reactome: R-HSA-6798695
Reactome: R-HSA-9018676
Reactome: R-HSA-9018681
Reactome: R-HSA-9018896
Reactome: R-HSA-9020265
Reactome: R-HSA-9023661
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Leukotriene A-4 Hydrolase antibody
Anti-Lta-4 Hydrolase antibody
Anti-Leukotriene A(4 Hydrolase antibody
Anti-Tripeptide Aminopeptidase Lta4h antibody
Anti-LTA4H antibody
Anti-LTA4 antibody

Information sourced from Uniprot.org

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