| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LTA4H (312-611) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LTA4H |
| Gene ID: |
4048 |
| Uniprot ID: |
LKHA4_HUMAN |
| Immunogen Region: |
312-611 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 312-611 of human LTA4H (NP_000886.1). |
| Immunogen Sequence: |
WDHFWLNEGHTVYLERHICG RLFGEKFRHFNALGGWGELQ NSVKTFGETHPFTKLVVDLT DIDPDVAYSSVPYEKGFALL FYLEQLLGGPEIFLGFLKAY VEKFSYKSITTDDWKDFLYS YFKDKVDVLNQVDWNAWLYS PGLPPIKPNYDMTLTNACIA LSQRWITAKEDDLNSFNATD LKDLSSHQLNEFLAQTLQRA PLPLGHIKRMQEVYNFNAIN NSEIRFRWLRLCIQSKWED |
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts. |
| Post Translational Modifications | Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity. |
| Function | Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions. |
| Protein Name | Leukotriene A-4 HydrolaseLta-4 HydrolaseLeukotriene A(4 HydrolaseTripeptide Aminopeptidase Lta4h |
| Database Links | Reactome: R-HSA-2142691Reactome: R-HSA-6798695Reactome: R-HSA-9018676Reactome: R-HSA-9018681Reactome: R-HSA-9018896Reactome: R-HSA-9020265Reactome: R-HSA-9023661 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Leukotriene A-4 Hydrolase antibodyAnti-Lta-4 Hydrolase antibodyAnti-Leukotriene A(4 Hydrolase antibodyAnti-Tripeptide Aminopeptidase Lta4h antibodyAnti-LTA4H antibodyAnti-LTA4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and LTA4H knockout (KO) 293T cells, using [KO Validated] LTA4H Rabbit polyclonal antibody (STJ11100815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89751/358228/STJ11100815_1__53554.1713122556.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LTA4H knockout (KO) 293T cells, using [KO Validated] LTA4H Rabbit polyclonal antibody (STJ11100815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and LTA4H knockout (KO) 293T cells, using [KO Validated] LTA4H Rabbit polyclonal antibody (STJ11100816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89752/358229/STJ11100816_1__79661.1713122557.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LTA4H knockout (KO) 293T cells, using [KO Validated] LTA4H Rabbit polyclonal antibody (STJ11100816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
