• Western blot analysis of extracts of various cell lines, using LSM4 antibody (STJ115549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using LSM4 rabbit polyclonal antibody (STJ115549) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using LSM4 rabbit polyclonal antibody (STJ115549) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using LSM4 antibody (STJ115549) at dilution of 1:50. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 1 Mu g LSM4 antibody (STJ115549). Western blot was performed from the immunoprecipitate using LSM4 antibody (STJ115549) at a dilution of 1:1000.

Anti-LSM4 antibody (1-139) (STJ115549)

SKU:
STJ115549

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LSM4 (1-139) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LSM4
Gene ID: 25804
Uniprot ID: LSM4_HUMAN
Immunogen Region: 1-139
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1).
Immunogen Sequence: MLPLSLLKTAQNHPMLVELK NGETYNGHLVSCDNWMNINL REVICTSRDGDKFWRMPECY IRGSTIKYLRIPDEIIDMVK EEVVAKGRGRGGLQQQKQQK GRGMGGAGRGVFGGRGRGGI PGTGRGQPEKKPGRQAGKQ
Function Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.
Protein Name U6 Snrna-Associated Sm-Like Protein Lsm4
Glycine-Rich Protein
Grp
Database Links Reactome: R-HSA-430039
Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-U6 Snrna-Associated Sm-Like Protein Lsm4 antibody
Anti-Glycine-Rich Protein antibody
Anti-Grp antibody
Anti-LSM4 antibody

Information sourced from Uniprot.org

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