• Western blot analysis of extracts from normal (control) and Lrwd1 knockout (KO) HeLa cells, using Lrwd1 antibody (STJ11100719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Lrwd1 antibody (250-350) (STJ11100719)

SKU:
STJ11100719

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Lrwd1 (250-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRWD1
Gene ID: 222229
Uniprot ID: LRWD1_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Lrwd1 (NP_690852.1).
Immunogen Sequence: ASPSAQVEGSPVAGSDGSQP AVKLEPLHFLQCHSKNNSPQ DLETQLWACAFEPAWEEGAT SQTVATCGGEAVCVIDCQTG IVLHKYKAPGEEFFSVAWTA L
Tissue Specificity Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS).
Post Translational Modifications Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation. Ubiquitination occurs within the WD repeats at the end of the G1 phase. Ubiquitination may be catalyzed by the CUL4-DDB1 E3 ubiquitin-protein ligase complex and other E3 ligases.
Function Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
Protein Name Leucine-Rich Repeat And Wd Repeat-Containing Protein 1
Centromere Protein 33
Cenp-33
Origin Recognition Complex-Associated Protein
Orc-Associated Protein
Orca
Cellular Localisation Nucleus
Chromosome
Centromere
Telomere
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Kinetochore
Localizes To Heterochromatin During G1 Phase
Restricted To Centromeres Or Telomeres As Cells Progress Though S Phase
When Cells Enter Mitosis
Relocalizes To Centromeres
Recruitment To Pericentric Heterochromatin Largely Depends On The Presence Of H3k9me3
Alternative Antibody Names Anti-Leucine-Rich Repeat And Wd Repeat-Containing Protein 1 antibody
Anti-Centromere Protein 33 antibody
Anti-Cenp-33 antibody
Anti-Origin Recognition Complex-Associated Protein antibody
Anti-Orc-Associated Protein antibody
Anti-Orca antibody
Anti-LRWD1 antibody
Anti-CENP-33 antibody
Anti-ORCA antibody

Information sourced from Uniprot.org

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