• Western blot analysis of extracts of mouse liver, using LRP6 antibody (STJ115287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LRP6 antibody (20-150) (STJ115287)

SKU:
STJ115287

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LRP6 (20-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRP6
Gene ID: 4040
Uniprot ID: LRP6_HUMAN
Immunogen Region: 20-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human LRP6 (NP_002327.2).
Immunogen Sequence: APLLLYANRRDLRLVDATNG KENATIVVGGLEDAAAVDFV FSHGLIYWSDVSEEAIKRTE FNKTESVQNVVVSGLLSPDG LACDWLGEKLYWTDSETNRI EVSNLDGSLRKVLFWQELDQ PRAIALDPSSG
Tissue Specificity Widely coexpressed with LRP5 during embryogenesis and in adult tissues.
Post Translational Modifications Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479. Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling. Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum. Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum. Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. N-glycosylation is required for cell surface location.
Function Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
Protein Name Low-Density Lipoprotein Receptor-Related Protein 6
Lrp-6
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Reactome: R-HSA-4641262
Reactome: R-HSA-4641263
Reactome: R-HSA-5340588
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum
Membrane Raft
On Wnt Signaling
Undergoes A Cycle Of Caveolin- Or Clathrin-Mediated Endocytosis And Plasma Membrane Location
Released From The Endoplasmic Reticulum On Palmitoylation
Mono-Ubiquitination Retains It In The Endoplasmic Reticulum In The Absence Of Palmitoylation
Phosphorylated
Aggregates And Colocalizes With Axin1 And Gsk3b At The Plasma Membrane In Lrp6-Signalosomes
Chaperoned To The Plasma Membrane By Mesd
Alternative Antibody Names Anti-Low-Density Lipoprotein Receptor-Related Protein 6 antibody
Anti-Lrp-6 antibody
Anti-LRP6 antibody

Information sourced from Uniprot.org

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