• Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa, using Lamin-B1 Rabbit monoclonal antibody (STJ119229) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.
  • Immunoprecipitation of Lamin B1 in 300 µg extracts from Jurkat cells using 0. 5 µg Lamin B1 Rabbit polyclonal antibody (STJ119229). Western blot analysis was performed using Lamin B1 Rabbit polyclonal antibody (STJ119229) at 1:1000 dilution.
  • Confocal immunofluorescence analysis of HeLa cells using Lamin B1 Rabbit polyclonal antibody (STJ119229) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 Rabbit polyclonal antibody (STJ119229) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Lamin B1 Rabbit polyclonal antibody (STJ119229) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Lamin B1 in paraffin-embedded mouse spinal cord using Lamin B1 Rabbit polyclonal antibody (STJ119229) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Lamin B1 in paraffin-embedded human breast cancer using Lamin B1 Rabbit polyclonal antibody (STJ119229) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Lamin B1 in paraffin-embedded rat kidney using Lamin B1 Rabbit polyclonal antibody (STJ119229) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Lamin B1 Rabbit polyclonal antibody (STJ119229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-LMNB1 antibody (397-586) (STJ119229)

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STJ119229

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LMNB1 (397-586) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB1
Gene ID: 4001
Uniprot ID: LMNB1_HUMAN
Immunogen Region: 397-586
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1).
Immunogen Sequence: RVTVSRASSSRSVRTTRGKR KRVDVEESEASSSVSISHSA SATGNVCIEEIDVDGKFIRL KNTSEQDQPMGGWEMIRKIG DTSVSYKYTSRYVLKAGQTV TIWAANAGVTASPPTDLIWK NQNSWGTGEDVKVILKNSQG EEVAQRSTVFKTTIPEEEEE EEEAAGVVVEEELFHQQGTP RASNRSCAIM
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Protein Name Lamin-B1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2559584
Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Reactome: R-HSA-352238
Reactome: R-HSA-4419969
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9013405
Reactome: R-HSA-9035034
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B1 antibody
Anti-LMNB1 antibody
Anti-LMN2 antibody
Anti-LMNB antibody

Information sourced from Uniprot.org

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