• Western blot analysis of extracts of various cell lines, using LLGL2 antibody (STJ110583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using LLGL2 antibody (STJ110583) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using LLGL2 antibody (STJ110583) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using LLGL2 antibody (STJ110583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-LLGL2 antibody (620-819) (STJ110583)

SKU:
STJ110583

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LLGL2 (620-819) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Llgl2
Gene ID: 217325
Uniprot ID: L2GL2_MOUSE
Immunogen Region: 620-819
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 620-819 of mouse LLGL2 (NP_663413.2).
Immunogen Sequence: RQVFVKCTLHPSDQLALEGP LSRVKSLKKSLRQSFRRMRR SRVSSHKRRPGGPTGEAQAQ AVNIKAERTGLQNMELAPVQ RKIEARSAEDSFTGFVRTLY FADTYLRDSSRHCPSLWAGT NGGTVYAFSLRVPPAERRTD EPVRAEQAKEIQLMHRAPVV GILVLDGHNVPLPEPLEVAH DLSKSPDMQGSHQLLVVSEE
Post Translational Modifications Phosphorylated at Ser-653 by PRKCI. Phosphorylation is enhanced during cell polarization induced by calcium. Phosphorylation may occur during the cell-cell contact-induced cell polarization and may contribute to the segregation of LLGL2 from the PRKCI/aPKC and PARD6B/Par-6 complex.
Function Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity.
Protein Name Llgl Scribble Cell Polarity Complex Component 2
Lethal Giant Larvae-Like Protein 2
Lethal(2 Giant Larvae Protein Homolog 2
Cellular Localisation Cytoplasm
Localized In The Perinuclear Structure And Faintly At The Cell-Cell Contacts Sites In The Interphase
Localized At The Cell Periphery During Metaphase
Cortical Localization In Mitotic Cells
Alternative Antibody Names Anti-Llgl Scribble Cell Polarity Complex Component 2 antibody
Anti-Lethal Giant Larvae-Like Protein 2 antibody
Anti-Lethal(2 Giant Larvae Protein Homolog 2 antibody
Anti-Llgl2 antibody
Anti-Llglh2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance