• Western blot analysis of mouse kidney, using Legumain (LGMN) antibody (STJ112585) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Legumain (LGMN) rabbit polyclonal antibody (STJ112585) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Legumain (Legumain (LGMN) ) Polyclonal Antibody (STJ112585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Legumain (Legumain (LGMN) ) Polyclonal Antibody (STJ112585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using Legumain (Legumain (LGMN) ) Polyclonal Antibody (STJ112585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-LGMN antibody (26-288) (STJ112585)

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STJ112585

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LGMN (26-288) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LGMN
Gene ID: 5641
Uniprot ID: LGMN_HUMAN
Immunogen Region: 26-288
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-288 of human Legumain (LGMN) (NP_005597.3?).
Immunogen Sequence: GGKHWVVIVAGSNGWYNYRH QADACHAYQIIHRNGIPDEQ IVVMMYDDIAYSEDNPTPGI VINRPNGTDVYQGVPKDYTG EDVTPQNFLAVLRGDAEAVK GIGSGKVLKSGPQDHVFIYF TDHGSTGILVFPNEDLHVKD LNETIHYMYKHKMYRKMVFY IEACESGSMMNHLPDNINVY ATTAANPRESSYACYYDEKR STYLGDWYSVNWMEDSDVED LTKETLHKQYHLVKSHTNT
Tissue Specificity Ubiquitous. Particularly abundant in kidney, heart and placenta.
Post Translational Modifications Activated by autocatalytic processing at pH 4.
Function Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.
Protein Name Legumain
Asparaginyl Endopeptidase
Aep
Protease - Cysteine 1
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-196791
Reactome: R-HSA-2132295
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Legumain antibody
Anti-Asparaginyl Endopeptidase antibody
Anti-Aep antibody
Anti-Protease - Cysteine 1 antibody
Anti-LGMN antibody
Anti-PRSC1 antibody

Information sourced from Uniprot.org

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