• Confocal imaging of Hep G2 cells using LDL Receptor (LDLR) Rabbit monoclonal antibody (STJ11103466, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of extracts of A-431 cells, using LDL Receptor (LDLR) antibody (STJ11103466) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using LDL Receptor (LDLR) antibody (STJ11103466) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-LDL Receptor antibody (761-860) [S6MR] (STJ11103466)

SKU:
STJ11103466

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LDL Receptor (LDLR) (761-860) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDLR
Gene ID: 3949
Uniprot ID: LDLR_HUMAN
Immunogen Region: 761-860
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Immunogen Sequence: TVEIVTMSHQALGDVAGRGN EKKPSSVRALSIVLPIVLLV FLCLGVFLLWKNWRLKNINS INFDNPVYQKTTEDEVHICH NQDGYSYPSRQMVSLEDDVA
Post Translational Modifications N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation.
Function Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Protein Name Low-Density Lipoprotein Receptor
Ldl Receptor
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8964026
Reactome: R-HSA-8964038
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane
Clathrin-Coated Pit
Golgi Apparatus
Early Endosome
Late Endosome
Lysosome
Rapidly Endocytosed Upon Ligand Binding
Alternative Antibody Names Anti-Low-Density Lipoprotein Receptor antibody
Anti-Ldl Receptor antibody
Anti-LDLR antibody

Information sourced from Uniprot.org

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