Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT1 antibody (545-644) [S7MR] (STJ11102547)

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STJ11102547
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT1
Gene ID: 3848
Uniprot ID: K2C1_HUMAN
Immunogen Region: 545-644
Specificity: A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (KRT1) (P04264).
Immunogen Sequence: GGGGRGSYGSGGSSYGSGGG SYGSGGGGGGHGSYGSGSSS GGYRGGSGGGGGGSSGGRGS GGGSSGGSIGGRGSSSGGVK SSGGSSSVKFVSTTYSGVTR
Tissue Specificity The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Post Translational Modifications Undergoes deimination of some arginine residues (citrullination).
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Protein Name Keratin - Type Ii Cytoskeletal 1
67 Kda Cytokeratin
Cytokeratin-1
Ck-1
Hair Alpha Protein
Keratin-1
K1
Type-Ii Keratin Kb1
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-9725554
Cellular Localisation Cell Membrane
Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 1 antibody
Anti-67 Kda Cytokeratin antibody
Anti-Cytokeratin-1 antibody
Anti-Ck-1 antibody
Anti-Hair Alpha Protein antibody
Anti-Keratin-1 antibody
Anti-K1 antibody
Anti-Type-Ii Keratin Kb1 antibody
Anti-KRT1 antibody
Anti-KRTA antibody

Information sourced from Uniprot.org

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