• Western blot analysis of extracts of various cell lines, using KIF2C/KIF2C/MCAK rabbit monoclonal antibody (STJ11102434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using KIF2C/KIF2C/MCAK Rabbit monoclonal antibody (STJ11102434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-KIF2C antibody (1-100) [S4MR] (STJ11102434)

SKU:
STJ11102434

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MCAK (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF2C
Gene ID: 11004
Uniprot ID: KIF2C_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIF2C/KIF2C/MCAK (Q99661).
Immunogen Sequence: MAMDSSLQARLFPGLAIKIQ RSNGLIHSANVRTVNLEKSC VSVEWAEGGATKGKEIDFDD VAAINPELLQLLPLHPKDNL PLQENVTIQKQKRRSVNSKI
Tissue Specificity Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary.expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
Post Translational Modifications Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. Ubiquitinated.
Function In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment.
Protein Name Kinesin-Like Protein Kif2c
Kinesin-Like Protein 6
Mitotic Centromere-Associated Kinesin
Mcak
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-6811434
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Chromosome
Centromere
Kinetochore
Associates With The Microtubule Network At The Growing Distal Tip (The Plus-End) Of Microtubules
Probably Through Interaction With Mtus2/Tip150 And Mapre1
Association With Microtubule Plus Ends Is Also Mediated By Interaction With Kif18b
Centromeric Localization Requires The Presence Of Bub1 And Sgo2
Alternative Antibody Names Anti-Kinesin-Like Protein Kif2c antibody
Anti-Kinesin-Like Protein 6 antibody
Anti-Mitotic Centromere-Associated Kinesin antibody
Anti-Mcak antibody
Anti-KIF2C antibody
Anti-KNSL6 antibody

Information sourced from Uniprot.org

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