![Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149736/302477/STJ16101020_1__58435.1646940657.jpg?c=1 "Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)")
![Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149736/302478/STJ16101020_2__70497.1646940657.jpg?c=1 "Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)")
![Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149736/302477/STJ16101020_1__58435.1646940657.jpg?c=1 "Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)")
| Host: | Mouse |
| Applications: | FC/WB/IF/IHC |
| Reactivity: | Cat/Dog/Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Keratin 13 + 10 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | EBS-IF-003 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (formalin-fixed: 2-4 |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A hybrid (H2d) mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion partner: Sp2/0. |
| Background | EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis. |
Information sourced from Uniprot.org