• Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)
  • Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)

Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)

SKU:
STJ16101020

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Host: Mouse
Applications: FC/WB/IF/IHC
Reactivity: Cat/Dog/Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin 13 + 10 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: EBS-IF-003
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (formalin-fixed: 2-4
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Immunogen: A hybrid (H2d) mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion partner: Sp2/0.
Background EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.

Information sourced from Uniprot.org

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