Host: |
Mouse |
Applications: |
FC/WB/IF/IHC |
Reactivity: |
Cat/Dog/Human |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Mouse monoclonal antibody anti-Keratin 13 + 10 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
Clonality: |
Monoclonal |
Clone ID: |
EBS-IF-003 |
Conjugation: |
Unconjugated |
Isotype: |
IgG2ak |
Formulation: |
PBS with 0.02% Sodium Azide. |
Purification: |
Affinity purified from tissue culture. |
Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
Dilution Range: |
Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (formalin-fixed: 2-4 |
Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
Immunogen: |
A hybrid (H2d) mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion partner: Sp2/0. |
Background | EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance