• Western blot analysis of extracts of various cell lines, using KEL antibody (STJ112155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Anti-KEL antibody (70-310) (STJ112155)

SKU:
STJ112155

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KEL (70-310) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KEL
Gene ID: 3792
Uniprot ID: KELL_HUMAN
Immunogen Region: 70-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-310 of human KEL (NP_000411.1).
Immunogen Sequence: QNCGPRPCETSVCLDLRDHY LASGNTSVAPCTDFFSFACG RAKETNNSFQELATKNKNRL RRILEVQNSWHPGSGEEKAF QFYNSCMDTLAIEAAGTGPL RQVIEELGGWRISGKWTSLN FNRTLRLLMSQYGHFPFFRA YLGPHPASPHTPVIQIDQPE FDVPLKQDQEQKIYAQIFRE YLTYLNQLGTLLGGDPSKVQ EHSSLSISITSRLFQFLRPL EQRRAQGKLFQMVTIDQLK
Tissue Specificity Expressed at high levels in erythrocytes and testis (in Sertoli cells), and, at lower levels, in skeletal muscle, tonsils (in follicular dendritic cells), lymph node, spleen and appendix (at protein level). Also expressed in many adult and fetal nonerythroid tissues, including brain, spleen, lymph nodes and bone marrow.
Post Translational Modifications N-glycosylated.
Function Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3, with a marked preference for EDN3.
Protein Name Kell Blood Group Glycoprotein
Cd Antigen Cd238
Database Links Reactome: R-HSA-375276
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Spans The Erythrocyte Membrane
And Is Attached To The Underlying Cytoskeleton
Alternative Antibody Names Anti-Kell Blood Group Glycoprotein antibody
Anti-Cd Antigen Cd238 antibody
Anti-KEL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance