• Western blot analysis of extracts of MCF7 cells, using KCNJ3 antibody (STJ114329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts of mouse brain, using KCNJ3 antibody (STJ114329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-KCNJ3 antibody (50-150) (STJ114329)

SKU:
STJ114329

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KCNJ3 (50-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNJ3
Gene ID: 3760
Uniprot ID: KCNJ3_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KCNJ3 (NP_002230.1).
Immunogen Sequence: NGRCNVQHGNLGSETSRYLS DLFTTLVDLKWRWNLFIFIL TYTVAWLFMASMWWVIAYTR GDLNKAHVGNYTPCVANVYN FPSAFLFFIETEATIGYGYR Y
Function This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium.as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.
Protein Name G Protein-Activated Inward Rectifier Potassium Channel 1
Girk-1
Inward Rectifier K(+ Channel Kir3.1
Potassium Channel - Inwardly Rectifying Subfamily J Member 3
Database Links Reactome: R-HSA-1296041
Reactome: R-HSA-997272
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-G Protein-Activated Inward Rectifier Potassium Channel 1 antibody
Anti-Girk-1 antibody
Anti-Inward Rectifier K(+ Channel Kir3.1 antibody
Anti-Potassium Channel - Inwardly Rectifying Subfamily J Member 3 antibody
Anti-KCNJ3 antibody
Anti-GIRK1 antibody

Information sourced from Uniprot.org

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