• Immunohistochemistry analysis of paraffin-embedded human tonsil using Kappa light chain Rabbit monoclonal antibody (STJ11102949) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Human plasma cells, using Kappa light chain antibody (STJ11102949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Kappa light chain antibody (1-117) [S9MR] (STJ11102949)

SKU:
STJ11102949

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Kappa light chain (1-117) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGKV1D-16Uniprot ID=KVD16_HUMAN"
Immunogen Region: 1-117
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human Kappa light chain (P01601).
Immunogen Sequence: MDMRVLAQLLGLLLLCFPGA RCDIQMTQSPSSLSASVGDR VTITCRASQGISSWLAWYQQ KPEKAPKSLIYAASSLQSGV PSRFSGSGSGTDFTLTISSL QPEDFATYYCQQYNSYP
Function V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Protein Name Immunoglobulin Kappa Variable 1d-16
Ig Kappa Chain V-I Region Hk146
Ig Kappa Chain V-I Region Hk189
Cellular Localisation Secreted
Cell Membrane
Alternative Antibody Names Anti-Immunoglobulin Kappa Variable 1d-16 antibody
Anti-Ig Kappa Chain V-I Region Hk146 antibody
Anti-Ig Kappa Chain V-I Region Hk189 antibody
Anti-IGKV1D-16 antibody

Information sourced from Uniprot.org

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